Coding

Part:BBa_K4891000:Design

Designed by: Ruyi Shi   Group: iGEM23_Guangzhou-MedX   (2023-06-13)


aroGfbr


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 241


Design Notes

There are three DAHP synthase in E. coli MG1655, and these enzymes are feedback-inhibited by L-phenylalanine, L-tyrosine and L-tryptophan. Therefore, AroGfbr usage could alleviate this effect.

Source

This gene is originated from Escherichia coli MG1655.

References